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      當前位置 > 產品目錄 > 試劑 > 細胞生物學試劑 > 重組LIF(白血病抑制因子)
      重組LIF(白血病抑制因子)
      英文名稱:Recombinant LIF (Leukemia Inhibitory Factor)總訪問:38386
      國產/進口:進口半年訪問:1521
      產地/品牌:美國/PeproTech產品類別:細胞生物學試劑
      規       格:10ug/100ug 最后更新:2022-8-17
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      背景:
      白血病抑制因子(Leukemia Inhitory Factor, LIF)是一種通過抑制自發分化而促使胚胎干細胞(ES)長期存活的淋巴因子。去除LIF將引起干細胞的分化,但干細胞仍可保持其增殖潛能和多能性。人LIF對人和小鼠細胞具有相同的生物活性。大腸桿菌(E.coli)表達的重組人LIF由非糖基化的單個肽鏈組成,含有180個氨基酸,分子量為19,717Da。

      bioLEAD LIF的優點:   

      1)      對于人和小鼠細胞的活性相同

      2)      ES(胚胎干細胞)培養測試,可長期保持干細胞的未分化狀態和種系傳遞能力

      3)      工作濃度與其它公司同為1000U/ml,因此可做無縫隙直接轉換

      4)      純度高于95%

      5)      價格便宜

      6)      保證活性,否則100%退款

       bioLEAD LIF的活性測試:  

       1. 胚胎干細胞(ES)培養測試:

      小鼠胚胎干細胞(W4)在添加重組人LIF (1000 Units/mL)的培養基中培養。傳代10(25)后,胚胎干細胞仍保持著正常的形態 (1)。核型分析結果顯示,80%的胚胎干細胞含有正常的40條染色體(2)。

       

       2. 長期培養的胚胎干細胞(ES)種系傳遞能力測試: 

      小鼠胚胎干細胞(W4)在含有白血病抑制因子(重組人LIF)的培養基中培養,傳代10(25)后顯微注射入C57/BL6小鼠的囊胚腔中,仍可生成雄性嵌合體小鼠,主要來源于胚胎干細胞(>95%,根據Agouti鼠毛色判斷)。將兩只雄性嵌合體小鼠與雌性C57BL/6J小鼠雜交,所有的子代(26只,4), 均攜帶W4 胚胎干細胞來源的Agouti鼠毛色基因。結果表明長期培養的胚胎干細胞(ES)具有種系傳遞能力。

      使用該產品的文獻:
      Rotter et al. Mutant p53 facilitates somatic cell reprogramming and augments the malignant potential of reprogrammed cells. J. Exp. Med.. 2010; 207: 2127-2140.  

      bioLEAD LIF的詳細信息
      Cat. No.: BL-Z00384

      Source: E. coli

      MW: 19,717 Da

      Description:
      Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, stem cell pluripotency control, bone and fat metabolism, mitogenesis of certain factor-dependent cell lines, and the promotion of megakaryocyte production in vivo. Mature human and murine mature LIFs exhibit 78% sequence identity at the amino acid control.Human LIF is equally active on both human and mouse cells. Murine LIF is approximately 1000 times less active on human cells than hLIF. Recombinant Human LIF produced in E. coli is a single, non-glycosylated, polypeptide chain containing 180 amino acids and having a molecular mass of 19,717 Da.

      Purity:
      Greater than 95% as determined by the following methods:
      (a) RP-HPLC analysis
      (b) Anion-exchange FPLC
      (c) Reducing and non-reducing SDS-PAGE silver-stained gel
      analysis

      Endotoxin Level:
      Less than 0.1 ng/ug (1 IEU/ug) of LIF

      Specific Activity:
      The ED50 is determined by the M1 cell differentiation assay is < 0.01 ng/ml, corresponding to a specific activity of > 1×108 units/mg.

      Storage:
      Lyophilized rHuLIF remains stable at room temperature for three weeks, but it is best stored desiccated below -18°C. Upon reconstitution rHuLIF should be stored at 4°C for up to one week and -18°C for longer periods it is recomme nded that a carrier protein (0.1% HSA or BSA) be added. Please avoid repeated freeze-thaw cycles

      Formulation:
      LIF is lyophilized after extensive dialysis against PBS.

      Reconstitution:
      We recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0 mg/ml. We also recommend that further dilutions be made into buffer or medium to which protein (e.g., 1% BSA) or Tween 20 has been added. This solution can then be stored at 4°C for one week or at -20°C for longer periods.

      Sequence analysis:
      The sequence of the first five N-terminal amino acids has been found to be Ser-Pro-Leu-Pro-Ile.

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